Molecular differences distinguish clonal lineages within East African populations of Fusarium oxysporum f.sp. Cubense

Lodwig, E.M. and Bridge, P.D. and Rutherford, M.A. and Kung'u, J.N. and Jeffries, P. (1999) Molecular differences distinguish clonal lineages within East African populations of Fusarium oxysporum f.sp. Cubense. Journal of Applied Microbiology, 86 (1). pp. 71-77. ISSN 1364-5072. (The full text of this publication is not available from this repository)

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Abstract

Molecular approaches for the assessment of intraspecific diversity within an economically important plant pathogen were compared with traditional physiological methods (vegetative compatibility testing). The vegetative compatibility groups (VCGs) of 14 isolates of Fusarium oxysporum f.sp. cubense (FOC) from Kenya were first assessed using nitrate non-utilizing mutants. Nine of these isolates, from different areas of the country, were compatible with one or more of VCGs 0124, 0125, 0128 and 01220, i.e. they formed a single clonal lineage. Three isolates, all originating from the banana growing district of Kisii, were compatible with the VCG 01212 and formed a second distinct clonal lineage. Mutants could not be recovered from one isolate (62) and two isolates (27 and 30) were not vegetatively compatible with any of the VCG testers and may represent two novel VCGs. Polymerase chain reaction (PCR) fingerprinting, especially when using the M13 derived primer, was found to produce banding patterns that correlated with clonal lineage and also distinguished isolates 27 and 30 when analysed by unweighted pair group method analysis and principle co-ordinate analysis. This approach also distinguished FOC from F. oxysporum IMI50438 isolated from Triticum sp. and from isolates of Colletotrichum gloeosporioides. Total protein profiles were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and although clonal lineages were not separated, isolates 27 and 30 were again distinguishable and FOC produced a different profile to F. oxysporum (IMI 350438) and C. gloeosporioides.

Item Type: Article
Subjects: Q Science > QR Microbiology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: I.T. Ekpo
Date Deposited: 08 Apr 2009 09:55
Last Modified: 31 Jul 2012 08:20
Resource URI: http://kar.kent.ac.uk/id/eprint/16906 (The current URI for this page, for reference purposes)
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