Efficient human IFN-gamma expression in the mammary gland of transgenic mice

Lagutin, O.V. and Dobrovolsky, V.N. and Vinogradova, T.V. and Kyndiakov, B.N. and Khodarovich, Y.M. and Jenkins, N. and James, D.C. and Markham, N. and Larionov, O.A. (1999) Efficient human IFN-gamma expression in the mammary gland of transgenic mice. Journal of Interferon and Cytokine Research, 19 (2). pp. 137-144. ISSN 1079-9907. (The full text of this publication is not available from this repository)

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Abstract

Two hybrid genes (BLG-HuIFN-gamma 2 and BLG-HuIFN-gamma 3) were constructed on the basis of sheep beta-lactoglobulin (BLG) and human interferon-gamma (HuIFN-gamma) gene sequences. They were used to direct HuIFN-gamma synthesis in the mammary gland of transgenic mice. HuIFN-gamma was efficiently produced in the mammary gland of transgenic mice. BLG-HuIFN-gamma 2 transgenic females expressed HuIFN-gamma in the milli at concentrations up to 570 mg/ml, and BLG-HuIFN-gamma 3 transgenic females expressed up to 350 mg/ml. All females carrying the BLG-HuIFN-gamma 3 gene expressed HuIFN-gamma in their milk. No significant changes were observed in the HuIFN-gamma expression level during the lactation period, Using RT-PCR analysis, ectopic expression for both hybrid genes was found in transgenic mice. Despite ectopic expression of HuIFN-gamma in transgenic mice, their development and pregnancy were normal. The heritability of the HuIFN-gamma expression lever in milk was demonstrated up to the F-2 generation. This work demonstrates that hybrid genes have the potential to develop in transgenic domestic animals producing HuIFN-gamma in milk.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: I.T. Ekpo
Date Deposited: 14 Apr 2009 15:14
Last Modified: 14 Apr 2009 15:14
Resource URI: http://kar.kent.ac.uk/id/eprint/16894 (The current URI for this page, for reference purposes)
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