Lagutin, O.V. and Dobrovolsky, V.N. and Vinogradova, T.V. and Kyndiakov, B.N. and Khodarovich, Y.M. and Jenkins, N. and James, D.C. and Markham, N. and Larionov, O.A. (1999) Efficient human IFN-gamma expression in the mammary gland of transgenic mice. Journal of Interferon and Cytokine Research, 19 (2). pp. 137-144. ISSN 1079-9907.
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Two hybrid genes (BLG-HuIFN-gamma 2 and BLG-HuIFN-gamma 3) were constructed on the basis of sheep beta-lactoglobulin (BLG) and human interferon-gamma (HuIFN-gamma) gene sequences. They were used to direct HuIFN-gamma synthesis in the mammary gland of transgenic mice. HuIFN-gamma was efficiently produced in the mammary gland of transgenic mice. BLG-HuIFN-gamma 2 transgenic females expressed HuIFN-gamma in the milli at concentrations up to 570 mg/ml, and BLG-HuIFN-gamma 3 transgenic females expressed up to 350 mg/ml. All females carrying the BLG-HuIFN-gamma 3 gene expressed HuIFN-gamma in their milk. No significant changes were observed in the HuIFN-gamma expression level during the lactation period, Using RT-PCR analysis, ectopic expression for both hybrid genes was found in transgenic mice. Despite ectopic expression of HuIFN-gamma in transgenic mice, their development and pregnancy were normal. The heritability of the HuIFN-gamma expression lever in milk was demonstrated up to the F-2 generation. This work demonstrates that hybrid genes have the potential to develop in transgenic domestic animals producing HuIFN-gamma in milk.
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||I.T. Ekpo|
|Date Deposited:||14 Apr 2009 15:14|
|Last Modified:||14 Apr 2009 15:14|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/16894 (The current URI for this page, for reference purposes)|
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