Comparison of the ability of dopamine receptor agonists to inhibit forskolin-stimulated adenosine 3 ' 5 '-cyclic monophosphate (cAMP) accumulation via D-2L (long isoform) and D-3 receptors expressed in Chinese hamster ovary (CHO) cells

Hall, David A. and Strange, Philip G. (1999) Comparison of the ability of dopamine receptor agonists to inhibit forskolin-stimulated adenosine 3 ' 5 '-cyclic monophosphate (cAMP) accumulation via D-2L (long isoform) and D-3 receptors expressed in Chinese hamster ovary (CHO) cells. Biochemical Pharmacology, 58 (2). pp. 285-289. ISSN 0006-2952. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1016/S0006-2952(99)00101-X

Abstract

The pharmacological properties of the human D-2L (long isoform) and rat D-3 dopamine receptors in functional assays were examined. A range of dopamine agonists were assessed for their ability to inhibit adenosine 3'5' cyclic monophosphate (cAMP) accumulation via the two receptors expressed stably in Chinese hamster ovary cells. Dopamine caused a significantly greater maximal inhibition (P < 0.05) of cAMP accumulation via the D-2L receptor (similar to 70%) as compared to the D-3 receptor (similar to 50%). The pattern of agonist effects was different at the two receptors. The absolute and relative potencies for inhibition of cAMP accumulation were different for a range of agonists acting at the two receptors. Similarly, the maximal inhibitions achieved by a range of agonists were different for the two receptors.

Item Type: Article
Uncontrolled keywords: dopamine; D-3 receptor; D-2L receptor; adenylyl cyclase
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: I.T. Ekpo
Date Deposited: 21 Jun 2009 21:51
Last Modified: 16 Apr 2014 15:44
Resource URI: http://kar.kent.ac.uk/id/eprint/16829 (The current URI for this page, for reference purposes)
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