Species and population structures of Pisolithus and Scleroderma identified by combined phenotypic and genomic marker analysis

Sims, Karen P. and Sen, Robin and Watling, Roy and Jeffries, Peter (1999) Species and population structures of Pisolithus and Scleroderma identified by combined phenotypic and genomic marker analysis. Mycological Research, 103 . pp. 449-458. ISSN 0953-7562. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1017/S095375629800728X

Abstract

Isolates of Pisolithus and Scleroderma species from different northern temperate and tropical geographical regions were subjected to analyses of pure culture morphology, colony growth rates, isozyme (allozyme) variation and ribosomal DNA (rDNA) restriction fragment length polymorphisms (RFLPs). Cultural characteristics enabled clear species separation of isolates and together with growth rates suggested geographically-linked intraspecific variability in the Pisolithus populations. Combined or method-specific hierarchical cluster analyses of allozyme polymorphisms and RFLPs of the fungal internal transcribed spacer (ITS) and intergenic spacer (IGS) sequences confirmed the Scleroderma species groupings and considerable geographical and host-linked variation in the Pisolithus population. Isolates of Pisolithus from the Philippines were genetically very homogeneous and distinct from less related isolates from Europe, Scandinavia and North America. Based on the ITS-RFLP and isozyme polymorphism data, the isolates investigated probably represent four different 'species groupings' which supports similar findings from recent taxonomic and genetic studies of Australian Pisolithus species.

Item Type: Article
Additional information: Part 4
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: F.D. Zabet
Date Deposited: 26 Mar 2009 11:15
Last Modified: 27 May 2014 10:37
Resource URI: http://kar.kent.ac.uk/id/eprint/16619 (The current URI for this page, for reference purposes)
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