The Candida albicans CUG-decoding ser-tRNA has an atypical anticodon stem-loop structure

Perreau, Victoria M. and Keith, Gerard and Holmes, W. Mike and Przykorska, Anna and Santos, Manuel and Tuite, Mick F. (1999) The Candida albicans CUG-decoding ser-tRNA has an atypical anticodon stem-loop structure. Journal of Molecular Biology, 293 (5). pp. 1039-1053. ISSN 0022-2836. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1006/jmbi.1999.3209

Abstract

In many Candida species, the leucine CUG codon is decoded by a tRNA with two unusual properties: it is a ser-tRNA and, uniquely, has guanosine at position 33 (G33). Using a combination of enzymatic (V-1, RNase, RnI nuclease) and chemical (Pb2+, imidazole) probing of the native Candida albicans ser-tRNA(CAG), we demonstrate that the overall tertiary structure of this tRNA resembles that of a ser-tRNA rather than a leu-tRNA, except within the anticodon arm where there is considerable disruption of the anticodon stem. Using non-modified in vitro transcripts of the C. albicans ser-tRNA(CAG), carrying G, C, U or A at position 33, we demonstrate that it is specifically a G residue at this position that induces the atypical anticodon stem structure. Further quantitative evidence for an unusual structure in the anticodon arm of the G33-tRNA is provided by the observed change in kinetics of methylation of the G at position 37, by purified Escherichia coli m(1)G37 methyltransferase. We conclude that the anticodon arm distortion, induced by a guanosine base at position 33 in the anticodon loop of this novel tRNA, results in reduced decoding ability which has facilitated the evolution of this tRNA without extinction of the species encoding it.

Item Type: Article
Uncontrolled keywords: Candida albicans; ser-tRNA(CAG); tRNA structure; structure probing; codon reassignment
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: F.D. Zabet
Date Deposited: 28 Apr 2009 19:03
Last Modified: 10 Jun 2014 12:46
Resource URI: http://kar.kent.ac.uk/id/eprint/16449 (The current URI for this page, for reference purposes)
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