Hypoxanthine transport and transient expression of possible regulatory proteins in COS-1 cells

Johnson, C. and Shayeghi, Majid and Jarvis, Simon M. (2000) Hypoxanthine transport and transient expression of possible regulatory proteins in COS-1 cells. International Congress Series, 1208 . pp. 329-330. ISSN 0531-5131. (The full text of this publication is not available from this repository)

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Abstract

We have demonstrated that monkey kidney (COS-1) cells have a single type of nucleobase transport process. The system is sodium-independent, and transport of hypoxanthine was insensitive to inhibition by nitrobenzylthioinosine (NBMPR), but was inhibited by nucleosides. These properties are consistent with hypoxanthine transport occurring via the NBMPR-insensitive nucleoside carrier (ei). Transfecting COS-1 cells with opossum kidney cDNA fractions resulted in similar to 70% inhibition of hypoxanthine influx suggesting the possible presence of genes within the library that act as apparent inhibitors of hypoxanthine transport. Further studies are required to clarify the nature of this inhibition, but the COS-1 expression system appears to be a useful tool for the possible identification of important regulatory proteins.

Item Type: Article
Additional information: Conference Information: 1st International Conference on Control and Diseases of Sodium Dependent Transport Proteins and Ion Channels SHIZUOKA, JAPAN, AUG 24-28, 1999 Pharmaceut Soc Japan; Physiol Soc Japan; Molec Biol Soc Japan; Biophys Soc Japan; Japanese Biochem Soc; Japanese Pharmacol Soc; Japanese Soc Toxicol Sci Proceedings Paper
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: O.O. Odanye
Date Deposited: 17 Apr 2009 14:18
Last Modified: 17 Jul 2014 14:03
Resource URI: http://kar.kent.ac.uk/id/eprint/16265 (The current URI for this page, for reference purposes)
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