Carbonic anhydrase inhibitors: Inhibition of the beta-class enzymes from the fungal pathogens Candida albicans and Cryptococcus neoformans with simple anions

Innocenti, Alessio and Mühlschlegel, Fritz A. and Hall, Rebecca A. and Steegborn, Clemens and Scozzafava, Andrea and Supuran, Claudiu T. (2008) Carbonic anhydrase inhibitors: Inhibition of the beta-class enzymes from the fungal pathogens Candida albicans and Cryptococcus neoformans with simple anions. Bioorganic & Medicinal Chemistry Letters, 18 (18). pp. 5066-5070. ISSN 0960-894X. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1016/j.bmcl.2008.07.122

Abstract

The catalytic activity and inhibition of the beta-carbonic anhydrases (CAs, EC 4.2.1.1) from the pathogenic fungi Candida albicans ( Nce103) and Cryptococcus neoformans (Can2) with inorganic anions such as halogenides, pseudohalogenides, bicarbonate, carbonate, nitrate, nitrite, hydrogen sulfide, bisulfite, perchlorate, sulfate were investigated. The two enzymes showed appreciable CO2 hydrase activity (k(cat) in the range of (3.9-8.0) x 10(5) s (1), and k(cat)/K-m in the range of (4.3-9.7) x 10(7) M (1) s (1)). Can2 was weakly inhibited by cyanide and sulfamic acid (K(I)s of 8.22-13.56 mM), while all other anions displayed more potent inhibition. Nce103 was strongly inhibited by cyanide and carbonate (K(I)s of 10-11 mu M), and weakly inhibited by sulfate, phenylboronic, and phenyl arsonic acid (K(I)s of 14.15-30.85 mM). These data demonstrate that pathogenic, fungal beta-CAs may be targets for the development of antifungals that have a novel mechanism of action.

Item Type: Article
Uncontrolled keywords: beta-carbonic anhydrases; anions; Candida albicans; Nce103; Cryptococcus neoformans; Can2; fungal pathogens
Depositing User: Jane Griffiths
Date Deposited: 22 Apr 2009 11:03
Last Modified: 09 Apr 2014 15:53
Resource URI: http://kar.kent.ac.uk/id/eprint/15708 (The current URI for this page, for reference purposes)
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