Sebastianelli, A. and Sen, T. and Bruce, I.J. (2008) Extraction of DNA from soil using nanoparticles by magnetic bioseparation. Letters in Applied Microbiology, 46 (4). pp. 488-491. ISSN 0266-8254.
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Aims: To develop a simple, rapid and inexpensive soil DNA extraction protocol. Methods and Results: The protocol relies on the use of superparamagnetic silica-magnetite nanoparticles for the isolation and purification of DNA from soil samples. DNA suitable for use in molecular biology applications was obtained from a number of soil samples. Conclusions: The DNA extracted using the tested method successfully permitted the PCR amplification of a fragment of the bacterial 16S rDNA gene. The extracted DNA could also be restriction endonuclease digested. Significance and Impact of the Study: The protocol reported here is simple and permits rapid isolation of PCR-ready soil DNA. The method requires only small quantities of soil sample, is scalable and suitable for automation.
|Uncontrolled keywords:||DNA; magnetic bioseparation; PCR; rapid techniques; soil|
|Subjects:||Q Science > QR Microbiology|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||Maureen Cook|
|Date Deposited:||03 Mar 2009 14:35|
|Last Modified:||03 Mar 2009 14:35|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/15658 (The current URI for this page, for reference purposes)|
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