Two distinct roles for two functional cobaltochelatases (CbiK) in Desulfovibrio vulgaris Hildenborough

Lobo, S.A.L. and Brindley, A.A. and Romao, C.V. and Leech, H.K. and Warren, M.J. and Saraiva, L.M. (2008) Two distinct roles for two functional cobaltochelatases (CbiK) in Desulfovibrio vulgaris Hildenborough. Biochemistry, 47 (21). pp. 5851-5857. ISSN 0006-2960. (The full text of this publication is not available from this repository)

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Abstract

The sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough possesses a large number of porphyrin-containing proteins whose biosynthesis is poorly characterized. In this work, we have studied two putative CbiK cobaltochelatases present in the genome of D. vulgaris. The assays revealed that both enzymes insert cobalt and iron into sirohydrochlorin, with specific activities with iron lower than that measured with cobalt. Nevertheless, the two D. vulgaris chelatases complement an E. coli cysG mutant strain showing that, in vivo, they are able to load iron into sirohydrochlorin. The results showed that the functional cobaltochelatases have distinct roles with one, CbiK(C), likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis, while the other, CbiK(P), is periplasmic located and possibly associated with an iron transport system. Finally, the ability of D. vulgaris to produce vitamin B-12 was also demonstrated in this work.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Maureen Cook
Date Deposited: 03 Sep 2009 11:10
Last Modified: 03 Sep 2009 11:10
Resource URI: http://kar.kent.ac.uk/id/eprint/15500 (The current URI for this page, for reference purposes)
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