tRNA and protein methylase complexes mediate zymocin toxicity in yeast

Studte, Patrick and Zink, Sabrina and Jablonowski, Daniel and Bar, Christian and von der Haar, Tobias and Tuite, Mick F. and Schaffrath, Raffael (2008) tRNA and protein methylase complexes mediate zymocin toxicity in yeast. Molecular Microbiology, 69 (5). pp. 1266-1277. ISSN 0950-382X. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1111/j.1365-2958.2008.06358.x

Abstract

Modification of Saccharomyces cerevisiae tRNA anticodons at the wobble uridine (U34) position is required for tRNA cleavage by the zymocin tRNase killer toxin from Kluyveromyces lactis. Hence, U34 modification defects including lack of the U34 tRNA methyltransferase Trm9 protect against tRNA cleavage and zymocin. Using zymocin as a tool, we have identified toxin-resistant mutations in TRM9 that are likely to affect the U34 methylation reaction. Most strikingly, C-terminal truncations in Trm9 abolish interaction with Trm112, a protein shown to individually purify with Lys9 and two more methylases, Trm11 and Mtq2. Downregulation of a GAL1-TRM112 allele protects against zymocin whereas LYS9, TRM11 and MTQ2 are dosage suppressors of zymocin. Based on immune precipitation studies, the latter scenario correlates with competition for Trm112 and in excess, some of these Trm112 partners interfere with formation of the toxin-relevant Trm9.Trm112 complex. In contrast to trm11 Delta or lys9 Delta cells, trm112 Delta and mtq2 Delta null mutants are zymocin resistant. In line with the identified role that methylation of Sup45 by Mtq2 has for translation termination by the release factor dimer Sup45.Sup35, we observe that SUP45 overexpression and sup45 mutants suppress zymocin. Intriguingly, this suppression correlates with upregulated levels of tRNA species targeted by zymocin's tRNase activity.

Item Type: Article
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Q Science > QR Microbiology
Q Science > QP Physiology (Living systems) > QP506 Molecular biology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Louise Dorman
Date Deposited: 03 Sep 2009 13:44
Last Modified: 10 Jun 2014 11:10
Resource URI: http://kar.kent.ac.uk/id/eprint/15241 (The current URI for this page, for reference purposes)
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