Anderson, S.J. and Gould, P. and Freeland, J.R. (2007) Repetitive flanking sequences (ReFS): novel molecular markers from microsatellite families. Molecular Ecology Notes, 7 (3). pp. 374-376. ISSN 1471-8278.
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Although microsatellite markers have become exceedingly popular in molecular studies of wild organisms, their development in some taxonomic groups is challenging. This is partly because of repetitive flanking sequences, which lead to the simultaneous amplification of alleles from multiple loci. Until now, these microsatellite DNA families have been considered unsuitable for population genetics studies, but here we describe our development of these repetitive flanking sequences (ReFS) as novel molecular markers. We illustrate the utility of these markers by using them to address an outstanding taxonomic question in the moth genus Schrankia.
|Uncontrolled keywords:||microsatellites; molecular markers; ReFS; repetitive DNA; Schrankia LEPIDOPTERA; DNA|
|Subjects:||G Geography. Anthropology. Recreation > GE Environmental Sciences|
|Divisions:||Faculties > Social Sciences > School of Anthropology and Conservation > Biodiversity Conservation Group|
|Depositing User:||Stephen Holland|
|Date Deposited:||19 Dec 2007 18:56|
|Last Modified:||14 Jan 2010 14:03|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/1439 (The current URI for this page, for reference purposes)|
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