Boussouf, S.E. and Geeves, M.A. (2007) Tropomyosin and troponin cooperativity on the thin filament. In: Regulatory Mechanisms of Striated Muscle Contraction. Advances in Experimental Medicine and Biology, 592. Springer-Verlag Berlin, Heidelberger Platz 3, D-14197 Berlin, Germany, Berlin, Germany pp. 99-109. ISBN 978-4-431-38451-9 .
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The regulation of muscle contraction by the thin filament proteins tropomyosin (Tm) and troponin (Tn) has remained an area of interest since the proteins were first discovered 40 years ago.1,2 Although we have learnt a great deal about the proteins themselves and the mechanism by which they regulate muscle contraction some aspects of the mechanism remain to be adequately explained. Our interest is in the cooperativity of the calcium regulatory process and this remains poorly understood and several different models have been proposed. At it simplest the essence of the problem can be simply outlined. In skeletal muscle the binding of calcium to the two regulatory sites of TnC is required for activation of muscle contraction. Isolated TnC binds the calcium cooperatively, as might be expected for a two-calcium-ion switch, with a hill coefficient (h) of between 1 & 2.3,4 In contrast the calcium activation of isometric force in a skinned muscle fibre occurs with a much larger hill coefficient5,6 leading to the idea that cooperativity extends beyond the single actin7TmTn structural unit of the thin filament. Some models of muscle activation suggest the whole filament switches as a single unit while studies of the purified proteins in solution tend to indicate more limited cooperativity extending to only the nearest neighbour actin7TmTn units. In this paper the reasons why the nature of the cooperativity remains a problem will be explored together with an overview of what our recent studies of the proteins in solution have revealed about thin filament cooperativity.
|Item Type:||Conference or workshop item (Paper)|
|Additional information:||This item is also chapter 10 of the book entitled " Regulatory Mechanisms of Striated Muscle Contraction". 0065-2598 (Print) Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||Michael Geeves|
|Date Deposited:||16 Aug 2009 18:23|
|Last Modified:||20 May 2011 23:50|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/13218 (The current URI for this page, for reference purposes)|
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