Nyitrai, M. and Szent-Gyorgyi, A.G. and Geeves, M.A. (2003) Interactions of the two heads of scallop (Argopecten irradians) heavy meromyosin with actin: influence of calcium and nucleotides. Biochem J, 370 (Pt 3). pp. 839-848. ISSN 0264-6021.
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We recently proposed a co-operative model for the influence of calcium and ADP on scallop ( Argopecten irradians ) muscle heavy meromyosin (scHMM), in which scHMM exists in two conformations (designated 'off' and 'on'), and calcium and ADP are allosteric effectors of the equilibrium between the off and on conformations [Nyitrai, Szent-Gyorgyi and Geeves (2002) Biochem. J. 365, 19-30]. Here we examine the influence of actin on scHMM. In the absence of nucleotide, both heads of scHMM bind very tightly to actin, independent of the presence of calcium. In the absence of calcium, ADP dissociates scHMM from actin completely, and little evidence of ternary complex formation can be found (actin affinity >20 microM). The off state of scHMM therefore does not interact with actin. In the presence of calcium, ADP and actin lower each other's affinity for scHMM by 30-50-fold, although both heads remain strongly attached to actin (actin affinity 0.17 microM). Detailed analysis suggests that the second head contributes far more to the overall binding energy than is the case for mammalian skeletal muscle HMM. This is consistent with a different stereochemical relationship between the two heads in scallop and mammalian HMM molecules.
|Additional information:||0264-6021 (Print) Journal Article|
|Uncontrolled keywords:||Actins/*metabolism Adenosine Diphosphate/metabolism Adenosine Triphosphate/metabolism Animals Calcium/*metabolism Mollusca/*chemistry Muscle, Skeletal/chemistry/metabolism Myosin Subfragments/*metabolism Nucleotides/*metabolism Protein Binding Protein Conformation Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Spectrometry, Fluorescence|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||Michael Geeves|
|Date Deposited:||14 Mar 2009 16:21|
|Last Modified:||14 Mar 2009 16:21|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/13195 (The current URI for this page, for reference purposes)|
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