Wu, Z. and Benfield, R.E. and Wang, Y. and Guo, L. and Tang, M. and Zhang, H. and Ge, Y. and Grandjean, D. (2001) EXAFS study on the local atomic structures around iron in glycosated haemoglobin. PHYSICS IN MEDICINE AND BIOLOGY , 46 (3). N71-N77. ISSN 0031-9155.
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Samples with 5.1%, 9.8% and 15.3% HbA1c were extracted from normal subjects and patients with slight and serious diabetes respectively. Extended x-ray absorption fine structure spectra of Fe K absorption were collected at the EXAFS experimental station of the Beijing Synchrotron Radiation Facility. The step-by-step fluorescent mode was employed with a count time of 10 s per point. Several independent scans were averaged to eliminate the statistical noise. Reference backscattering amplitudes and phaseshifts were calculated using the curve wave theory (FEFF code) of EXAFS. Apart from the nitrogen neighbours around the central iron atom, oxygen neighbours are also found. The Fe-N bond length increases by about 0.02 Angstrom for the sample with 15.3% HbA1c compared with the others, but the Fe-O bond length is almost unchanged. With increasing of HbA1c concentration, the content of Hb increases and the content of HbO(2) decreases. This demonstrates that the glycosylation of haemoglobin will decrease its ability to carry oxygen.
|Subjects:||Q Science > QD Chemistry
Q Science > QC Physics
|Divisions:||Faculties > Science Technology and Medical Studies > School of Physical Sciences|
|Depositing User:||Robert E Benfield|
|Date Deposited:||09 Oct 2008 15:04|
|Last Modified:||09 Oct 2008 15:04|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/12705 (The current URI for this page, for reference purposes)|
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