Nuclear organization in human sperm: preliminary evidence for altered sex chromosome centromere position in infertile males

Finch, Katie A. and Fonseka, K.G.L. and Abogrein, A. and Ioannou, Dimitris and Handyside, Alan H and Thornhill, Alan R. and Hickson, Nicholas and Griffin, Darren K. (2008) Nuclear organization in human sperm: preliminary evidence for altered sex chromosome centromere position in infertile males. Human Reproduction, 23 (6). pp. 1263-1270. ISSN 0268-1161. (The full text of this publication is not available from this repository)

The full text of this publication is not available from this repository. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1093/humrep/den112

Abstract

BACKGROUND: Many genetic defects with a chromosomal basis affect male reproduction via a range of different mechanisms. Chromosome position is a well-known marker of nuclear organization, and alterations in standard patterns can lead to disease phenotypes such as cancer, laminopathies and epilepsy. It has been demonstrated that normal mammalian sperm adopt a pattern with the centromeres aligning towards the nuclear centre. The purpose of this study was to test the hypothesis that altered chromosome position in the sperm head is associated with male infertility. METHODS: The average nuclear positions of fluorescence in-situ hybridization signals for three centromeric probes (for chromosomes X, Y and 18) were compared in normoozoospermic men and in men with compromised semen parameters. RESULTS: In controls, the centromeres of chromosomes X, Y and 18 all occupied a central nuclear location. In infertile men the sex chromosomes appeared more likely to be distributed in a pattern not distinguishable from a random model. CONCLUSIONS: Our findings cast doubt on the reliability of centromeric probes for aneuploidy screening. The analysis of chromosome position in sperm heads should be further investigated for the screening of infertile men.

Item Type: Article
Additional information: den112 1460-2350
Uncontrolled keywords: male infertility; sperm; centromere; FISH
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Darren Griffin
Date Deposited: 27 Mar 2009 18:09
Last Modified: 04 Jul 2014 13:34
Resource URI: http://kar.kent.ac.uk/id/eprint/12498 (The current URI for this page, for reference purposes)
  • Depositors only (login required):