Finch, K.A. and Fonseka, K.G.L. and Abogrein, A. and Ioannou, D. and Handyside, A.H. and Thornhill, A.R. and Hickson, N. and Griffin, D.K. (2008) Nuclear organization in human sperm: preliminary evidence for altered sex chromosome centromere position in infertile males. Human Reproduction (Oxford, England), 23 (6). pp. 1263-1270. ISSN 0268-1161 .
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BACKGROUND: Many genetic defects with a chromosomal basis affect male reproduction via a range of different mechanisms. Chromosome position is a well-known marker of nuclear organization, and alterations in standard patterns can lead to disease phenotypes such as cancer, laminopathies and epilepsy. It has been demonstrated that normal mammalian sperm adopt a pattern with the centromeres aligning towards the nuclear centre. The purpose of this study was to test the hypothesis that altered chromosome position in the sperm head is associated with male infertility. METHODS: The average nuclear positions of fluorescence in-situ hybridization signals for three centromeric probes (for chromosomes X, Y and 18) were compared in normoozoospermic men and in men with compromised semen parameters. RESULTS: In controls, the centromeres of chromosomes X, Y and 18 all occupied a central nuclear location. In infertile men the sex chromosomes appeared more likely to be distributed in a pattern not distinguishable from a random model. CONCLUSIONS: Our findings cast doubt on the reliability of centromeric probes for aneuploidy screening. The analysis of chromosome position in sperm heads should be further investigated for the screening of infertile men.
|Additional information:||den112 1460-2350|
|Uncontrolled keywords:||male infertility; sperm; centromere; FISH|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||Darren Griffin|
|Date Deposited:||27 Mar 2009 18:09|
|Last Modified:||27 Mar 2009 18:09|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/12498 (The current URI for this page, for reference purposes)|
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