Harvey, S.C. and Boonphakdee, C. and Campos-Ramos, R. and Ezaz, M.T. and Griffin, D.K. and Bromage, N.R. and Penman, P. (2003) Analysis of repetitive DNA sequences in the sex chromosomes of Oreochromis niloticus. Cytogenetic and Genome Research, 101 (3-4). pp. 314-319. ISSN 1424-8581 .
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In the Nile tilapia, Oreochromis niloticus, sex determination is primarily genetic, with XX females and XY males. While the X and Y chromosomes (the largest pair) cannot be distinguished in mitotic chromosome spreads, analysis of comparative hybridization of X and Y chromosome derived probes (produced, by microdissection and DOP-PCR, from XX and YY genotypes, respectively) to different genotypes (XX, XY and YY) has demonstrated that sequence differences exist between the sex chromosomes. Here we report the characterization of these probes, showing that a significant proportion of the amplified sequences represent various transposable elements. We further demonstrate that concentrations of a number of these individual elements are found on the sex chromosomes and that the distribution of two such elements differs between the X and Y chromosomes. These findings are discussed in relation to sex chromosome differentiation in O. niloticus and to the changes expected during the early stages of sex chromosome evolution.
|Additional information:||14685001 1424-859X|
|Uncontrolled keywords:||Animals Chromosome Mapping Chromosome Painting Cichlids DNA Transposable Elements Evolution, Molecular Female Male Models, Genetic Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Sex Chromosomes|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||Darren Griffin|
|Date Deposited:||10 Sep 2008 10:59|
|Last Modified:||03 Mar 2009 11:49|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/12479 (The current URI for this page, for reference purposes)|
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