Harvey, S.C. and Masabanda, J. and Carrasco, L.A.P. and Bromage, N.R. and Penman, D.J. and Griffin, D.K. (2002) Molecular-cytogenetic analysis reveals sequence differences between the sex chromosomes of Oreochromis niloticus: evidence for an early stage of sex-chromosome differentiation. Cytogenetic and Genome Research, 97 (1-2). pp. 76-80. ISSN 1424-8581.
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Sex determination in the Nile tilapia, Oreochromis niloticus, is primarily genetic, with XX females and XY males. A candidate sex-determining region in the terminal region of the largest chromosome pair has been identified by analysis of meiotic chromosomes. This region shows an inhibition of pairing and synapsis in the XY genotype, but not in XX or YY genotypes, suggesting that recombination is inhibited. Here we show that chromosome microdissection and subsequent amplification by degenerate oligonucleotide-primed PCR (DOP-PCR) can be used to produce in situ hybridization probes to this largest pair of O. niloticus chromosomes. Furthermore, analysis of the comparative hybridization of X and Y chromosome-derived probes to different genotypes provides the first demonstration that sequence differences exist between the sex chromosomes of O. niloticus. This provides further support for the theory that this chromosome pair is related to sex determination and further suggests that the sex chromosomes are at a very early stage of divergence.
|Additional information:||12438743 1424-8581|
|Uncontrolled keywords:||Animals Chromosome Painting Cichlids Cytogenetics DNA Probes Female In Situ Hybridization, Fluorescence Male Molecular Biology Sex Determination (Genetics) Sex Differentiation X Chromosome Y Chromosome|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||Darren Griffin|
|Date Deposited:||24 Sep 2008 12:38|
|Last Modified:||03 Mar 2009 12:00|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/12471 (The current URI for this page, for reference purposes)|
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