Roessner, C.A. and Huang, K.X. and Warren, M.J. and Raux, E. and Scott, A.I. (2002) Isolation and characterization of 14 additional genes specifying the anaerobic biosynthesis of cobalamin (vitamin B12) in Propionibacterium freudenreichii (P. shermanii). Microbiology, 148 (6). pp. 1845-53. ISSN 1350-0872 .
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A search for genes encoding enzymes involved in cobalamin (vitamin B12) production in the commercially important organism Propionibacterium freudenreichii (P. shermanii) has resulted in the isolation of an additional 14 genes encoding enzymes responsible for 17 steps of the anaerobic B12 pathway in this organism. All of the genes believed to be necessary for the biosynthesis of adenosylcobinamide from uroporphyrinogen III have now been isolated except two (cbiA and an as yet unidentified gene encoding cobalt reductase). Most of the genes are contained in two divergent operons, one of which, in turn, is closely linked to the operon encoding the B12-dependent enzyme methylmalonyl-CoA mutase. The close linkage of the three genes encoding the subunits of transcarboxylase to the hemYHBXRL gene cluster is reported. The functions of the P. freudenreichii B12 pathway genes are discussed, and a mechanism for the regulation of cobalamin and propionic acid production by oxygen in this organism is proposed.
|Additional information:||1350-0872 (Print) Journal Article Research Support, U.S. Gov't, P.H.S.|
|Uncontrolled keywords:||Anaerobiosis Base Sequence Biological Transport Cloning, Molecular Cobalt/metabolism Consensus Sequence Genes, Bacterial/*genetics Methyltransferases/genetics Molecular Sequence Data Operon/genetics Propionibacterium/enzymology/*genetics/*metabolism Uroporphyrinogens/metabolism Vitamin B 12/*analogs & derivatives/*biosynthesis/metabolism|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||Martin Warren|
|Date Deposited:||12 Oct 2009 07:15|
|Last Modified:||14 Jan 2010 14:42|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/11057 (The current URI for this page, for reference purposes)|
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