Erskine, P.T. and Coates, L. and Butler, D. and Youell, J.H. and Brindley, A.A. and Wood, S.P. and Warren, M.J. and Shoolingin-Jordan, P.M. and Cooper, J.B. (2003) X-ray structure of a putative reaction intermediate of 5-aminolaevulinic acid dehydratase. Biochemical Journal , 373 (Pt 3). pp. 733-8. ISSN 0264-6021 .
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The X-ray structure of yeast 5-aminolaevulinic acid dehydratase, in which the catalytic site of the enzyme is complexed with a putative cyclic intermediate composed of both substrate moieties, has been solved at 0.16 nm (1.6 A) resolution. The cyclic intermediate is bound covalently to Lys(263) with the amino group of the aminomethyl side chain ligated to the active-site zinc ion in a position normally occupied by a catalytic hydroxide ion. The cyclic intermediate is catalytically competent, as shown by its turnover in the presence of added substrate to form porphobilinogen. The findings, combined with those of previous studies, are consistent with a catalytic mechanism in which the C-C bond linking both substrates in the intermediate is formed before the C-N bond.
|Uncontrolled keywords:||Crystallography, X-Ray Models, Molecular Porphobilinogen Synthase/*chemistry/metabolism Protein Structure, Tertiary Recombinant Proteins/chemistry/metabolism Saccharomyces cerevisiae/enzymology|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||Martin Warren|
|Date Deposited:||09 Oct 2009 08:04|
|Last Modified:||14 Jan 2010 14:42|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/11052 (The current URI for this page, for reference purposes)|
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