Cantilever sensor for nanomechanical detection of specific protein conformations.

Mukhopodhyay, R. and Sumbayev, V. and Lorentzen, M. and Kjems, J. and Andreasen, P.A. and Besenbacher, F. (2005) Cantilever sensor for nanomechanical detection of specific protein conformations. NANO Letters, 5 (12). pp. 2385-2388. ISSN 1530-6984 . (The full text of this publication is not available from this repository)

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Official URL
http://pubs.acs.org/doi/abs/10.1021/nl051449z

Abstract

A fast, label-free, and multiplexed method based on piezoresistive cantilevers is reported for the detection of specific protein conformations at the nanoscale level. The ligand-binding domain of the human oestrogen receptor (ER alpha-LBD) is used as the experimental model system, and ER alpha-LBD with or without oestradiol (E2) is detected using the conformation-specific peptides alpha I beta II (Ser-Ser-Asn-His-Gin-Ser-Ser-Arg-Leu-IIe-Glu-Leu-Leu-Ser-Arg, which recognizes E2-bound ER) and alpha I beta II (Ser-Ala-Pro-Arg-Ala-Thr-lie-Ser-His-Tyr-Leu-Met-Gly-Gly, which recognizes E2-free ER). Target-specific signals are obtained in situ at protein concentrations of 2.5-20 nM. The in-build electrical readout of the piezoresistive cantilevers provides a convenient alternative to the conventional optical detection, and the presented method offers the possibility of detecting protein conformational changes using miniaturized microarrays.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > University wide - Teaching/Research Groups
Depositing User: Vadim Sumbayev
Date Deposited: 02 Apr 2009 20:22
Last Modified: 14 Jan 2010 14:38
Resource URI: http://kar.kent.ac.uk/id/eprint/10035 (The current URI for this page, for reference purposes)
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