Mukhopodhyay, R. and Sumbayev, V. and Lorentzen, M. and Kjems, J. and Andreasen, P.A. and Besenbacher, F. (2005) Cantilever sensor for nanomechanical detection of specific protein conformations. NANO Letters, 5 (12). pp. 2385-2388. ISSN 1530-6984 .
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| Official URL http://pubs.acs.org/doi/abs/10.1021/nl051449z |
Abstract
A fast, label-free, and multiplexed method based on piezoresistive cantilevers is reported for the detection of specific protein conformations at the nanoscale level. The ligand-binding domain of the human oestrogen receptor (ER alpha-LBD) is used as the experimental model system, and ER alpha-LBD with or without oestradiol (E2) is detected using the conformation-specific peptides alpha I beta II (Ser-Ser-Asn-His-Gin-Ser-Ser-Arg-Leu-IIe-Glu-Leu-Leu-Ser-Arg, which recognizes E2-bound ER) and alpha I beta II (Ser-Ala-Pro-Arg-Ala-Thr-lie-Ser-His-Tyr-Leu-Met-Gly-Gly, which recognizes E2-free ER). Target-specific signals are obtained in situ at protein concentrations of 2.5-20 nM. The in-build electrical readout of the piezoresistive cantilevers provides a convenient alternative to the conventional optical detection, and the presented method offers the possibility of detecting protein conformational changes using miniaturized microarrays.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science |
| Divisions: | Faculties > University wide - Teaching/Research Groups |
| Depositing User: | Vadim Sumbayev |
| Date Deposited: | 02 Apr 2009 20:22 |
| Last Modified: | 14 Jan 2010 14:38 |
| Resource URI: | http://kar.kent.ac.uk/id/eprint/10035 (The current URI for this page, for reference purposes) |
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